Conference Abstract Book

Cell Free Solution of LAB strains Preparetion of CFS 1. İ noculum of (1%) indicator bacteria 2. 1. Centrufuged 3000 xg for 10 min 2. Filtreted For Derivasition Added 2 ml TCA 5 ml Broth Culture 1. Incubation in MRS at 37 o C 2. Sterilized by Filitration LAB strain in MRS Control 25% 50% Incubation at 37 o C 72 h. 10 ml LDB 5 ml CFS + 5ml LDB 2.5ml CFS + 7.5ml LDB 3. Extraction