Conference Abstract Book

Curcumin extraction & core preparation • WPN formation: WPI (50 mg/mL) was fully hydrated, adjusted to pH 2.0 , then heated at 85 °C for ~5 h to induce fibrillation; samples were analyzed under reducing SDS-PAGE (12% gel) to confirm conversion. • On the gel (user image), the WPN lane shows depletion of native WPI bands (e.g., β-LG/α-LA region) and an enrichment of low-molecular-weight peptides (~10–15 kDa) —consistent with acid-heat hydrolysis preceding fibril assembly 6